General characteristics of micro-cirganlim; Classification, Morphology, Physiology, Growths, Nutrition and Reproduction; Microbial growth
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Question No. 1
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What is the size range of most bacteria?
A.
0.01-0.1 micrometers
B.
10-100 micrometers
C.
0.5-2.0 micrometers
D.
100-1000 micrometers
Question No. 2
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Which staining technique is used to differentiate bacteria into two major groups?
A.
Ziehl-Neelsen staining
B.
Gram staining
C.
India ink staining
D.
Acid-fast staining
Question No. 3
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What is the primary component of bacterial cell wall?
A.
Peptidoglycan
B.
Chitin
C.
Phospholipids
D.
Cellulose
Question No. 4
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Which of the following shapes is NOT a common bacterial morphology?
A.
Bacilli (rod-shaped)
B.
Cocci (spherical)
C.
Cuboid
D.
Spirochete (spiral)
Question No. 5
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What is the most common method of reproduction in bacteria?
A.
Conjugation
B.
Binary fission
C.
Fragmentation
D.
Sexual reproduction
Question No. 6
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Which microorganism lacks a cell wall?
A.
Bacillus
B.
Streptococcus
C.
Mycobacterium
D.
Mycoplasma
Question No. 7
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What is the G+C content range in bacterial DNA?
A.
15-25% mol
B.
25-80% mol
C.
5-15% mol
D.
85-95% mol
Question No. 8
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Which phase of bacterial growth curve shows the maximum rate of cell division?
A.
Death phase
B.
Lag phase
C.
Stationary phase
D.
Exponential (Log) phase
Question No. 9
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What is the function of FtsZ protein in bacterial cell division?
A.
Flagella assembly
B.
Septum formation
C.
Cell wall synthesis
D.
DNA replication
Question No. 10
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Which type of microscopy is used to observe motility in unstained bacterial preparations?
A.
Phase contrast microscopy
B.
Electron microscopy
C.
Fluorescence microscopy
D.
Bright-field microscopy (hanging drop)
Question No. 11
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What type of nutrition do most bacteria exhibit?
A.
Photosynthetic
B.
Chemosynthetic only
C.
Heterotrophic
D.
Mixotrophic
Question No. 12
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Which bacterial group is characterized by high G+C content and filamentous growth?
A.
Actinobacteria
B.
Proteobacteria
C.
Firmicutes
D.
Bacteroidetes
Question No. 13
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What is the minimum time required for
E. coli
to complete one cell cycle under optimal conditions?
A.
2 hours
B.
5 minutes
C.
12 hours
D.
20 minutes
Question No. 14
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Which staining method is specifically used to identify mycobacteria?
A.
Ziehl-Neelsen (acid-fast) staining
B.
Simple staining
C.
Negative staining
D.
Gram staining
Question No. 15
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What are protozoa primarily classified as?
A.
Prokaryotic organisms
B.
Unicellular eukaryotic organisms
C.
Multicellular organisms
D.
Viruses
Question No. 16
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During which growth phase do bacteria synthesize new enzymes and essential metabolites?
A.
Stationary phase
B.
Lag phase
C.
Exponential phase
D.
Death phase
Question No. 17
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Which of the following is a characteristic feature of fungi?
A.
Possess rigid cell walls
B.
Photosynthetic organisms
C.
Lack nucleus
D.
Always unicellular
Question No. 18
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What is the typical width range of fungal hyphae?
A.
100-200 µm
B.
20-50 µm
C.
0.1-1 µm
D.
2-10 µm
Question No. 19
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Which term describes the bacterial growth in a closed system with no food added or waste removed?
A.
Open culture
B.
Batch culture
C.
Continuous culture
D.
Fed-batch culture
Question No. 20
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What is the reproduction method in yeasts?
A.
Spore formation only
B.
Fragmentation
C.
Binary fission
D.
Budding
Question No. 21
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Which bacterial structure is responsible for motility?
A.
Pili
B.
Flagella
C.
Capsule
D.
Fimbriae
Question No. 22
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What type of organism is classified under Firmicutes?
A.
High G+C Gram-positive bacteria
B.
Archaea
C.
Low G+C Gram-positive bacteria
D.
Gram-negative bacteria
Question No. 23
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Which microscopic technique uses dark-ground illumination to observe spirochetes?
A.
Electron microscopy
B.
Phase contrast microscopy
C.
Fluorescence microscopy
D.
Dark-field microscopy
Question No. 24
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What happens to the bacterial population during the stationary phase?
A.
All cells form spores
B.
No change - cell division equals cell death
C.
Rapid increase in cell number
D.
Complete cessation of metabolism
Question No. 25
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Which technique is used to demonstrate bacterial capsules?
A.
Gram staining
B.
Methylene blue staining
C.
Acid-fast staining
D.
Negative staining with India ink
Question No. 26
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What is the primary genetic material in viruses?
A.
Either DNA or RNA
B.
Only DNA
C.
Only RNA
D.
Proteins only
Question No. 27
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Which bacterial group includes organisms with internal flagella and tight spiral shape?
A.
Firmicutes
B.
Actinobacteria
C.
Proteobacteria
D.
Spirochaetes
Question No. 28
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What is measured to determine bacterial growth in microbial populations?
A.
Individual cell size only
B.
Rate of metabolism only
C.
Increase in population number or overall mass
D.
Oxygen consumption only
Question No. 29
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Which process is NOT a method of asexual reproduction in bacteria?
A.
Meiosis
B.
Binary fission
C.
Multiple fission
D.
Budding
Question No. 30
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What characterizes organisms in the death or decline phase?
A.
Formation of endospores only
B.
Stable cell population
C.
Rapid cell multiplication
D.
Exponential decrease in viable cells
Question No. 31
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Which microorganism group is characterized as strict intracellular parasites?
A.
Mycoplasma
B.
Actinomyces
C.
Chlamydiae
D.
Spirochaetes
Question No. 32
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What is the purpose of generation time (
g
) calculation in microbiology?
A.
To measure metabolic rate
B.
To measure cell size
C.
To identify bacterial species
D.
To determine time for population doubling
Question No. 33
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Which component differentiates prokaryotic cells from eukaryotic cells?
A.
Ability to reproduce
B.
Presence of cytoplasm
C.
Presence of ribosomes
D.
Absence of membrane-bound nucleus
Question No. 34
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What is the primary mode of nutrition in fungi?
A.
Chemosynthesis
B.
Absorption of soluble nutrients
C.
Photosynthesis
D.
Ingestion of solid particles
Question No. 35
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Which bacterial arrangement results from division in irregular planes?
A.
Diplococci
B.
Clusters (Staphylococci)
C.
Chains (Streptococci)
D.
Tetrads
Question No. 36
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What is the function of peptidoglycan in bacterial cells?
A.
Genetic material
B.
Protein synthesis
C.
Structural support and cell wall formation
D.
Energy storage
Question No. 37
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Which taxonomic rank is used immediately before species in bacterial classification?
A.
Family
B.
Order
C.
Class
D.
Genus
Question No. 38
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What metabolite is commonly produced during the stationary phase?
A.
Secondary metabolites like antibiotics
B.
Nucleotides
C.
Primary metabolites
D.
Fatty acids only
Question No. 39
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Which nucleic acid sequencing is most commonly used for bacterial phylogenetic analysis?
A.
16S rRNA
B.
5S rRNA
C.
tRNA
D.
23S rRNA
Question No. 40
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What is the typical size range of protozoa?
A.
1-5 mm
B.
2-100 µm
C.
200-500 µm
D.
0.1-1 µm
Question No. 41
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Which bacterial structure protects DNA from damage during starvation?
A.
Plasmid
B.
Spore coat
C.
DPS (DNA-binding proteins from starved cells)
D.
Capsule
Question No. 42
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What characterizes dimorphic fungi?
A.
Always yeast-like
B.
Mycelial in culture, yeast-like in infected lesions
C.
Lack cell walls
D.
Always filamentous
Question No. 43
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Which technique amplifies DNA for bacterial identification?
A.
Western blotting
B.
ELISA
C.
PCR (Polymerase Chain Reaction)
D.
Southern blotting
Question No. 44
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What is the G+C content percentage similarity required for species differentiation?
A.
75%
B.
100%
C.
Less than 97%
D.
50%
Question No. 45
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Which bacterial group is known for producing mycolic acids?
A.
Bacillus
B.
Streptococcus
C.
Mycobacterium
D.
Clostridium
Question No. 46
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What is oligotrophic environment?
A.
Acidic environment
B.
Low-nutrient environment
C.
High-temperature environment
D.
High-nutrient environment
Question No. 47
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Which staining reaction characterizes
Treponema
?
A.
Gram-positive
B.
No staining reaction
C.
Gram-negative
D.
Acid-fast positive
Question No. 48
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What is the primary difference between moulds and yeasts?
A.
Presence of cell wall
B.
Reproduction method
C.
Filamentous vs. unicellular growth
D.
Nutrition mode
Question No. 49
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Which bacterial morphology is described as arc-shaped?
A.
Spirillum
B.
Bacilli
C.
Vibrio
D.
Cocci
Question No. 50
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What is the main feature of bacterial growth in exponential phase?
A.
Cell death equals cell division
B.
Predictable population doubling
C.
Cells are adapting to medium
D.
Nutrient depletion
Question No. 51
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Which bacterial component is targeted by lysozyme enzyme?
A.
Ribosomes
B.
Peptidoglycan in cell wall
C.
DNA
D.
Cell membrane
Question No. 52
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What type of reproduction occurs in protozoa?
A.
Budding only
B.
Only sexual reproduction
C.
Both asexual (schizogony) and sexual (sporogony)
D.
Only asexual reproduction
Question No. 53
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Which bacterial structure is composed of lipoprotein and derived from host cell?
A.
Capsule
B.
Pili
C.
Cell wall
D.
Viral envelope
Question No. 54
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What is the Adansonian system of taxonomy based on?
A.
Gram staining only
B.
Morphology only
C.
Statistical coefficient considering all characters equally
D.
DNA sequencing
Question No. 55
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Which bacterial group includes organisms capable of endospore formation?
A.
Streptococcus
B.
Neisseria
C.
Escherichia
D.
Bacillus and Clostridium
Question No. 56
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What is a characteristic feature of Proteobacteria?
A.
Lack peptidoglycan
B.
High G+C Gram-positive
C.
Obligate anaerobes only
D.
Gram-negative bacteria
Question No. 57
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Which technique uses fluorescent dyes to detect bacteria directly in specimens?
A.
Simple staining
B.
India ink staining
C.
FISH (Fluorescence in-situ hybridization)
D.
Gram staining
Question No. 58
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What happens to bacilli during stationary phase physiological changes?
A.
They elongate further
B.
They lose cell wall
C.
They form spores immediately
D.
They become almost spherical
Question No. 59
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Which microorganism group feeds by ingesting solid particles?
A.
Fungi
B.
Protozoa
C.
Bacteria
D.
Viruses
Question No. 60
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What is the basis for MALDI-TOF mass spectrometry in bacterial identification?
A.
Unique mass spectra from charged macromolecules
B.
Motility patterns
C.
Antibiotic resistance
D.
DNA sequencing
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